ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of thymidylate synthase (TS) gene polymorphisms on high-dose methotrexate (HD-MTX)-related toxicities in childhood acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>A total of 73 children who were diagnosed with ALL between March 2011 and March 2013 were included into this study. Genomic DNAs were extracted from their peripheral blood. And then the genotypes of TS 5'-UTR were determined by direct DNA sequencing after PCR. The toxicity response of 73 patients receiving HD-MTX chemotherapy were observed and recorded, and plasma MTX concentrations at 42-48 hours after chemotherapy were measured.</p><p><b>RESULTS</b>The main HD-MTX-related toxicities of 73 patients receiving HD-MTX chemotherapy were neutropenia, decreased hemoglobin level, thrombocytopenia, liver toxicity, mucosal damage, and gastrointestinal reactions. There were no significant differences in the incidence rate of HD-MTX-related toxicities between children with different TS 5'-UTR genotypes after chemotherapy (P>0.05). TS 5'-UTR genotype was not significantly correlated with plasma MTX concentrations at 42-48 hours after chemotherapy (P>0.05).</p><p><b>CONCLUSIONS</b>TS gene polymorphisms have no influence on the incidence of HD-MTX-related toxicities in childhood ALL.</p>
Subject(s)
Child , Child, Preschool , Female , Humans , Infant , Male , Antimetabolites, Antineoplastic , Genotype , Methotrexate , Polymorphism, Genetic , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Drug Therapy , Genetics , Thymidylate Synthase , GeneticsABSTRACT
Objective To evaluate the effect of community health self-management which was carried out by health club. Methods A total of 163 health club members attended 6 lessons of standardized health self-management course during 3 months. The awareness rate of related healthy knowledge,self-management behavior forming rate,BMI,blood pressure, FBG and other indexes were measured before and after 3 months. Results The average awareness rate increased significantly after the project(P<0. 05). In terms of self-management behavior forming condition,the achievement rates significantly increased in aspects of"Daily exercise time longer than 30 minutes"and"Daily intake of vegetables more than 250g"(both P<0. 05). While the average diastolic pressure among 121 hypertensive participants and fasting blood -glucose value of 47 diabetic participants both decreased significantly after the project(both P<0. 05). Conclusion The effect of education on community health self-management for chronic disease prevention which carried out by health club is positive apparently. It has certain functions on blood pressure and FBG control while short-term influence on BMI has not been found.